Purified Rabbit anti-Carbonic Anhydrase 3 Antibody

SKU: DL96724A

Size 1: 100 ul

Synonyms : Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III; CA-III

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Carbonic Anhydrase 3

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Carbonic Anhydrase 3

WB description: Western blot analysis of Carbonic Anhydrase 3 expression in mouse kidney (A), mouse lung (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Carbonic Anhydrase 3 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Carbonic Anhydrase 3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 761

Gene ID (Mouse): 12350

Gene ID(Rat): 54232

SwissProt (Human): P07451

SwissProt (Mouse): P16015

SwissProt (Rat): P14141

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.