Purified Rabbit anti-BCKDK Antibody

SKU: DL91467A

Size 1: 100 ul

Synonyms : [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, Mk, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to BCKDK

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BCKDK. The exact sequence is proprietary.

WB description: Western blot analysis of BCKDK expression in Raji (A), HeLa (B), Jurkat (C), SHSY5Y (D) whole cell lysates.

IHC description: Immunohistochemical analysis of BCKDK staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of BCKDK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 10295

Gene ID (Mouse): 12041

Gene ID(Rat): 29603

SwissProt (Human): O14874

SwissProt (Mouse): O55028

SwissProt (Rat): Q00972

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.