Purified Rabbit anti-AMPK alpha 1/2 Antibody

SKU: DL90925A

Size 1: 100 ul

Synonyms : PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to AMPK alpha 1/2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AMPK alpha 1/2. The exact sequence is proprietary.

WB description: Western blot analysis of AMPK alpha 1/2 expression in HeLa (A), mouse brain (B), rat brain (C) whole cell lysates.

IHC description: Immunohistochemical analysis of AMPK alpha 1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5562; 5563

Gene ID (Mouse): 105787; 108079

Gene ID(Rat): 65248; 78975

SwissProt (Human): Q13131; P54646

SwissProt (Mouse): Q5EG47; Q8BRK8

SwissProt (Rat): P54645; Q09137

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.