Purified Rabbit anti-Alpha-tubulin 4a Antibody

SKU: DL96028A

Size 1: 100 ul

Synonyms : TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Alpha-tubulin 4a

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Alpha-tubulin 4a

WB description: Western blot analysis of Alpha-tubulin 4a expression in HepG2 (A), MCF7 (B), mouse heart (C), mouse brain (D), PC12 (E) whole cell lysates.

IHC description: Immunohistochemical analysis of Alpha-tubulin 4a staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Alpha-tubulin 4a staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/25 - 1/50), IF/IC (1/25 - 1/100)

Gene ID(Human): 7277

Gene ID (Mouse): 22145

Gene ID(Rat): 316531

SwissProt (Human): P68366

SwissProt (Mouse): P68368

SwissProt (Rat): Q5XIF6

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.