Purified Rabbit anti-Alpha-actin Antibody

SKU: DL94414A

Size 1: 100 ul

Synonyms : ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Alpha-actin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-actin. The exact sequence is proprietary.

WB description: Western blot analysis of Alpha-actin expression in mouse heart (A), mouse lung (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Alpha-actin staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 70

Gene ID (Mouse): 11464

Gene ID(Rat): 29275

SwissProt (Human): P68032

SwissProt (Mouse): P68033

SwissProt (Rat): P68035

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.