Purified Rabbit anti-Adenosine A2a Receptor Antibody

SKU: DL94930A

Size 1: 100 ul

Synonyms : ADORA2; Adenosine receptor A2a

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, D

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Adenosine A2a Receptor

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Adenosine A2a Receptor. The exact sequence is proprietary.

WB description: Western blot analysis of Adenosine A2a Receptor expression in Hela (A), HepG2 (B), NIH3T3 (C), H9C2 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Adenosine A2a Receptor staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Adenosine A2a Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 135

Gene ID (Mouse): 11540

Gene ID(Rat): 25369

SwissProt (Human): P29274

SwissProt (Mouse): Q60613

SwissProt (Rat): P30543

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.