Purified Mouse anti-alpha Smooth Muscle Actin Antibody

SKU: DL99622A

Size 1: 100 ul

Synonyms : ACTSA; ACTVS; Actin aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

host: Mouse

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: IH

Background: Mouse monoclonal antibody to alpha Smooth Muscle Actin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human alpha Smooth Muscle Actin. The exact sequence is proprietary.

WB description:

IHC description: Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human cardiac muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description: Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human liomyoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

FC description: Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Monoclonal

Conjugation:

Buffer: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Dilution: IH (1/100 - 1/300)

Gene ID(Human): 59

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P62736

SwissProt (Mouse):

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.