PTH ELISA kit (Rat)

SKU: CEA866Ra

Size: 96Tests

# of Times Cited in literature: 13

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: PTH

Target Full Name: Parathyroid Hormone

Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

Target Species: Rat

Uniprot: P04089

Gene ID: 24694

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Rat PTH / Parathyroid Hormone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 9.88-800pg/mL

Sensitivity: 4.39pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH). No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Parathyroid Hormone (PTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Parathyroid Hormone (PTH) and unlabeled Parathyroid Hormone (PTH) (Standards or samples) with the pre-coated antibody specific to Parathyroid Hormone (PTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample.

Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;

References Citing This Product: Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient Diet

Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized Rats

Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parameters

The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat

Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats.

Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia?

Effect of the “protein diet” and bone tissue.

Effect of whole body vibration on healthy rat plasma parameters

The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized Rats

Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized Rat

Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats

Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat

PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES

Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats