PTH ELISA kit (Human)

SKU: CEA866Hu

Size: 96Tests

# of Times Cited in literature: 4

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: PTH

Target Full Name: Parathyroid Hormone

Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

Target Species: Human

Uniprot: P01270

Gene ID: 5741

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human PTH / Parathyroid Hormone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 2.47-200pg/mL

Sensitivity: 0.91pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH). No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Parathyroid Hormone (PTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Parathyroid Hormone (PTH) and unlabeled Parathyroid Hormone (PTH) (Standards or samples) with the pre-coated antibody specific to Parathyroid Hormone (PTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample.

Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;

References Citing This Product: Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets

An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer

Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism

CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblasts

Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease