Porcine Cytochrome P450 7A1,CYP7A1 ELISA KIT

SKU: E0351Po

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives. {ECO:0000250|UniProtKB:P22680}.

Research_area:

Applications:ELISA

Target_protein:CYP7A1

Reactivity:Porcine

Assay type:Sandwich

Sensitivity:1.04ng/ml

Detection range:1.88-120ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:cytochrome P450 7A1

Species:Porcine

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O46491

MASS(Da):56846

GeneID:

Synonyms:CYP7A1 CYP7

Gene names:CYP7A1

Standard solution:240ng/ml