PLGF ELISA kit (Human)

SKU: SEA114Hu

Size: 96Tests

# of Times Cited in literature: 10

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: PLGF

Target Full Name: Placenta Growth Factor

Alternative Names: PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein

Target Species: Human

Uniprot: P49763

Gene ID: 5228

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human PLGF / Placenta Growth Factor

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 15.6-1,000pg/mL

Sensitivity: 6.5pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Placenta Growth Factor (PLGF). No significant cross-reactivity or interference between Placenta Growth Factor (PLGF) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Growth Factor (PLGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placenta Growth Factor (PLGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placenta Growth Factor (PLGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placenta Growth Factor (PLGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;Reproductive science;

References Citing This Product: Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle

Elevated PLGF contributes to small-cell lung cancer brain metastasis

Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis

Serum angiogenic profile in abnormal placentation

Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study

Relationship between elevated serum level of placental growth factor and status of gestational diabetes mellitus

sFlt-1/PlGF Ratio in Prediction of Short-Term Neonatal Outcome of Small for Gestational Age Neonates

Puerarin Reduces Radiation-Induced Vascular Endothelial Cell Damage Via miR-34a/Placental Growth Factor