GeneBio Systems

PINP ELISA kit (Human)

Name: PINP ELISA kit (Human)
Brand: GeneBio Systems
SKU: CEA957Hu
Price: 165700 JPY
Availability: InStock

SKU:CEA957Hu

¥165,700 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 34

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: PINP

Target Full Name: Procollagen I N-Terminal Propeptide

Alternative Names: P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Target Species: Human

Uniprot: P02452

Gene ID: 1277

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human PINP / Procollagen I N-Terminal Propeptide

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 2.47-200ng/mL

Sensitivity: 0.94ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide (PINP). No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen I N-Terminal Propeptide (PINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen I N-Terminal Propeptide (PINP) and unlabeled Procollagen I N-Terminal Propeptide (PINP) (Standards or samples) with the pre-coated antibody specific to Procollagen I N-Terminal Propeptide (PINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen I N-Terminal Propeptide (PINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen I N-Terminal Propeptide (PINP) in the sample.

Research Area: Metabolic pathway;Hepatology;Bone metabolism;

References Citing This Product: Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population

Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy

Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners

The effect of calcium and vitamin D supplementation on bone health of male Jockeys.

Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity.

Erratum to: Effects on bone metabolism markers and arterial stiffness by switching to rivaroxaban from warfarin in patients with atrial fibrillation

A Study Protocol: Spinal Morphology, Physical Performance, Quality of Life and Biochemical Markers in Adults at Risk of Osteoporotic Fractures

Periostin and sclerostin levels in juvenile Paget's disease

Congenital Hypothyroidism and Bone Remodeling Cycle

Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus

12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis.