Phospho-Smad1 (Ser214) Antibody , Cat. # AF8058

SKU: AF8058

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:SMAD1

GeneID:4086

Uniprot:Q15797

Specificity:Phospho-Smad1 (Ser214) Antibody detects endogenous levels of Smad1 only when phosphorylated at Ser214.

Immunogen:A synthesized peptide derived from human Smad1 around the phosphorylation site of Ser214.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Application:WB,IF/ICC,ELISA(peptide)

Dilution:WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2840121

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF8058-BP

Des# Blocking Peptide:Phospho-Smad1 (Ser214) Blocking peptide

Blocking Peptide Price:$549 CAD