Phospho-p27 Kip1 (Ser178) Antibody , Cat. # AF8052

SKU: AF8052

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:CDKN1B

GeneID:1027

Uniprot:P46527

Specificity:Phospho-p27 Kip1 (Ser178) Antibody detects endogenous levels of p27 Kip1 only when phosphorylated at Ser178.

Immunogen:A synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Ser178.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Bovine,Sheep,Rabbit,Dog

Application:WB,IHC,IF/ICC,ELISA(peptide)

Dilution:WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2840115

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF8052-BP

Des# Blocking Peptide:Phospho-p27 Kip1 (Ser178) Blocking peptide

Blocking Peptide Price:$549 CAD