Phospho-ERK1/2 (Thr202+Tyr204/Thr185+Tyr187) Antibody , Cat. # AF8208

SKU: AF8208

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:MAPK3,MAPK1

GeneID:5595,5594

Uniprot:P27361/P28482

Specificity:Phospho-ERK1/2 (Thr202+Tyr204/Thr185+Tyr187) Antibody detects endogenous levels of ERK1/2 only when phosphorylated at Thr202+Tyr204/Thr185+Tyr187.

Immunogen:A synthesized peptide derived from human ERK1/2 around the phosphorylation site of Thr202+Tyr204/Thr185+Tyr187.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat,Monkey

Prediction:Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit

Application:WB,IHC,ELISA(peptide)

Dilution:WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2840270

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF8208-BP

Des# Blocking Peptide:Phospho-ERK1/2 (Thr202+Tyr204/Thr185+Tyr187) Blocking peptide

Blocking Peptide Price:$549 CAD