Phospho-BUB3 (Tyr207) Antibody - Cat. #: CSB-PA117544

SKU: CSB-PA117544

Size :100ul

Clone Number:

Aliases:Aa2-050 antibody; AU019800 antibody; AU021329 antibody; AU043350 antibody; AW146323 antibody; BUB 3 antibody; BUB 3L antibody; BUB3 (budding uninhibited by benzimidazoles 3; yeast) homolog antibody; bub3 antibody; BUB3 budding uninhibited by benzimidazoles 3 homolog antibody; BUB3 mitotic checkpoint protein antibody; BUB3_HUMAN antibody; BUB3L antibody; Budding uninhibited by benomyl antibody; Budding uninhibited by benzimidazoles 3 antibody; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody; Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody; Budding uninhibited by benzimidazoles 3 homolog antibody; Budding uninhibited by benzimidazoles 3; S. cerevisiae; homolog of antibody; C78067 antibody; hBUB3 antibody; Mitotic checkpoint component antibody; Mitotic checkpoint protein bub3 antibody; OTTHUMP00000020679 antibody; OTTHUMP00000020680 antibody; WD repeat type I transmembrane protein A72.5 antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:O43684

Immunogen:Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y(p)-L-D) derived from Human Bub3.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB; WB:1:500-1:1000

Background:

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

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Seeley T.W., Wang L., Zhen J.Y.Biochem. Biophys. Res. Commun. 257:589-595(1999)

Tang Z., Shu H., Oncel D., Chen S., Yu H.Mol. Cell 16:387-397(2004)

Logarinho E., Resende T., Torres C., Bousbaa H.Mol. Biol. Cell 19:1798-1813(2008)

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Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:BUB3

Research Areas:Others