PGE1(Prostaglandin E1) ELISA Kit

SKU: ELK9815

Size:96T

Reactivity:General

Alternative Names:Prostaglandin E1; PGE1

Assay Type:Competitive Inhibition

Sensitivity:9.56 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine

Assay length:2h

Research Area:Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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