PE-Cy7 Anti-Human CD49d Antibody(HP1/2)

SKU: PC7-30309

Size: 20T

Target: Human CD49d

Form: liquid

Conjugation: PE-Cy7

Conjugation Information: PE-Cy7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).

Purification: 0

Purity: 0

Host: Mouse

Clonality: Monoclonal

Clone ID: HP1/2

Isotype: IgG2a, κ

Storage: PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.

Immunogen: 0

Synonyms: CD49 antigen-like family member D|CD49d|ITGA4|Integrin alpha-4|Integrin alpha-IV|VLA-4 subunit alpha

Observed MW:

Uniprot ID: P13612

Reactivity: Human

Tested Application: FC

Recommended dilution: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

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Gene ID: 3676

Research Area:

Catagory: Flow cytometry Antibodies