GeneBio Systems

NES1 ELISA kit (Human)

Name: NES1 ELISA kit (Human)
Brand: GeneBio Systems
SKU: CEA242Hu
Price: 165700 JPY
Availability: InStock

SKU:CEA242Hu

¥165,700 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 23

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: NES1

Target Full Name: Nesfatin 1

Alternative Names: -

Target Species: Human

Uniprot: P80303

Gene ID: 4925

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human NES1 / Nesfatin 1

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 617.3-50,000pg/mL

Sensitivity: 234.2pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nesfatin 1 (NES1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nesfatin 1 (NES1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Nesfatin 1 (NES1). No significant cross-reactivity or interference between Nesfatin 1 (NES1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Nesfatin 1 (NES1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Nesfatin 1 (NES1) and unlabeled Nesfatin 1 (NES1) (Standards or samples) with the pre-coated antibody specific to Nesfatin 1 (NES1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Nesfatin 1 (NES1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Nesfatin 1 (NES1) in the sample.

Research Area: -

References Citing This Product: Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder

High plasma nesfatin-1 level in patients with major depressive disorder

Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus

Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder

Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder

Decreased serum nesfatin-1 levels in endometriosis

Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.

Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome

The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.

The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers

New hormones to predict the severity of gallstone-induced acute pancreatitis