Mouse TRAF6(TNF Receptor Associated Factor 6) Microsample ELISA Kit

SKU: ELK10900MS

Size:96T

Reactivity:Mouse

Alternative Names:RNF85; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer

Assay Type:Sandwich

Sensitivity:90.7 ng/mL

Standard:20 µg/mL

Detection range:0.32-20 µg/mL

Sample type:Tissue homogenates, cell lysates and other biological fluids.

Assay length:3.5h

Research Area:Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRAF6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRAF6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRAF6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRAF6 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P70196