Mouse TGFb2(Transforming Growth Factor Beta 2) ELISA Kit

SKU: ELK5216

Size:96T

Reactivity:Mouse

Alternative Names:TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor

Assay Type:Competitive Inhibition

Sensitivity:8.95 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:Serum, plasma, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Cytokine;Infection immunity;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TGFb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P27090