Mouse NLRP3 Recombinant Protein (N-His)

SKU: MV001012

Size :50ug

Form:Lyophilized

Storage buffer:Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Purity:>90% as determined by SDS-PAGE.

Applications:ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Expression system:E. coli

Accession:Q8R4B8

Protein length:Ser715-Trp1033

Type:Recombinant

Stability and Storage:Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Reconstitution:Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.

Alternative Names:NACHT, LRR and PYD domains-containing protein 3, 3.6.4.-, Cold autoinflammatory syndrome 1 protein homolog, Cryopyrin, Mast cell maturation-associated-inducible protein 1, PYRIN-containing APAF1-like protein 1, Nlrp3, Cias1, Mmig1, Nalp3, Pypaf1

Species:Mus musculus (Mouse)

Shipping:In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Predicted molecular weight:37.45 kDa

Note:For research use only.