Mouse JUNB(Jun B Proto Oncogene) ELISA Kit

SKU: ELK6076

Size:96T

Reactivity:Mouse

Alternative Names:JUNB

Assay Type:Sandwich

Sensitivity:0.078 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse JUNB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse JUNB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse JUNB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse JUNB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P09450