Mouse Hypoxia-inducible Factor 1Α, HIF-1A ELISA Kit

SKU: E0294Mo

Size:96 Tests-1 Kit

Background:Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed: 26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:HIF1A

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.153ng/L

Detection range:0.3-90ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Hypoxia-Inducible Factor 1 alpha

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q61221

MASS(Da):93,516

GeneID:15251

Synonyms:ARNT-interacting protein;HIF 1A;HIF1A;HIF1-alpha;HIF-1-alpha;Hypoxia-inducible factor 1-alpha

Gene names:HIF1A

Standard solution:96ng/L