Mouse Hormone-sensitive Lipase, LIPE ELISA Kit

SKU: E2138Mo

Size:96 Tests-1 Kit

Background:Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed: 15550674, PubMed: 20625037, PubMed: 21454566, PubMed: 23066022, PubMed: 23291629). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and of the fatty acid (FA) esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Preferentially hydrolyzes FA esters at the sn-3 position of the glycerol backbone in DAGs (PubMed: 23066022). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (PubMed: 20625037, PubMed: 21454566). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity).

Research_area:Metabolism

Applications:ELISA

Target_protein:LIPE

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.024U/mL

Detection range:0.05-20U/mL

Sample type:Serum, plasma or other biological fluids

Full product name:Hormone-Sensitive Lipase

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P54310

MASS(Da):83,348

GeneID:16890

Synonyms:Hormone-sensitive lipase;HSL;LIPE;Monoacylglycerol lipase LIPE;REH;Retinyl ester hydrolase

Gene names:LIPE

Standard solution:24U/mL