Mouse Forkhead Box O3, FOXO3 ELISA Kit

SKU: E0997Mo

Size:96 Tests-1 Kit

Background:Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed: 18054316, PubMed: 18054315, PubMed: 23805378). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (PubMed: 18054316, PubMed: 18054315, PubMed: 25402684). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (By similarity). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (By similarity). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed: 23283301). Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (PubMed: 32103177).

Research_area:Others

Applications:ELISA

Target_protein:FOXO3

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.061ng/ml

Detection range:0.1-40ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Forkhead Box O3

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9WVH4

MASS(Da):71,064

GeneID:56484

Synonyms:Forkhead box protein O3;FOXO 3;FOXO3

Gene names:FOXO3

Standard solution:48ng/ml