Mouse Cytochrome P450 7A1,CYP7A1 ELISA KIT

SKU: E1933Mo

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (By similarity). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (Probable). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (By similarity). {ECO:0000250|UniProtKB:P22680, ECO:0000305|PubMed:14522988}.

Research_area:

Applications:ELISA

Target_protein:Cyp7a1

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:9.62ng/L

Detection range:20-6000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cholesterol 7-alpha-monooxygenase

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q64505

MASS(Da):57262

GeneID:13122

Synonyms:Cyp7a1 Cyp7

Gene names:Cyp7a1

Standard solution:6400ng/L