Mouse CPB1(Carboxypeptidase B1, Tissue) ELISA Kit

SKU: ELK10808

Size:96T

Reactivity:Mouse

Alternative Names:CPB; PCPB; PASP; Pancreas-specific protein

Assay Type:Sandwich

Sensitivity:0.26 ng/mL

Standard:50 ng/mL

Detection range:0.79-50 ng/mL

Sample type:Serum and plasma.

Assay length:3.5h

Research Area:Enzyme & Kinase;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CPB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CPB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CPB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CPB1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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