Mouse ASRGL1(L-asparaginase) ELISA Kit

SKU: ELK9901

Size:96T

Reactivity:Mouse

Alternative Names:ALP; ALP1; CRASH; asparaginase and isoaspartyl peptidase 1; ASRGL1

Assay Type:Sandwich

Sensitivity:0.122 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Serum, plasma, saliva and other biological fluids.

Assay length:3.5h

Research Area:Enzyme & Kinase;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ASRGL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ASRGL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ASRGL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ASRGL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q8C0M9