Mouse Anti-IAA(Anti-Insulin Autoantibody) ELISA Kit

SKU: ELK0650

Size:96T

Reactivity:Mouse

Alternative Names:Anti-Insulin; Antiinsulin; Insulin Antibodies

Assay Type:Sandwich

Sensitivity:0.32 ng/mL

Standard:50 ng/mL

Detection range:0.78-50 ng/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Signal transduction;Endocrinology;Autoimmunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve.

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