MMP1 (Cleaved-Ile271) Rabbit Polyclonal Antibody

SKU: BT-AP03630

Size:100μL

Background:Catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus| at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.|cofactor:Binds 2 zinc ions per subunit.|cofactor:Binds 4 calcium ions per subunit.|The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion| thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.|There are two distinct domains in this protein; the catalytic N-terminal| and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).|enzyme regulation:Can be activated without removal of the activation peptide.|Cleaves collagens of types I| II| and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection| interacts and cleaves the secreted viral Tat protein| leading to a decrease in neuronal Tat's mediated neurotoxicity.|online information:Collagenase entry|PTM:Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.|Belongs to the peptidase M10A family.|Contains 4 hemopexin-like domains.|subunit:Interacts with HIV-1 Tat.|

Research_area:Cancer; Cardiovascular; Cell biology; Signal transduction

Target_protein:MMP1

applications:WB, ELISA

Reactivity:Human, Rat, Mouse

Clonality:Polyclonal

Clone ID:

Host:Rabbit

Isotype:IgG

Gene Symbol/ Name:MMP1

Immunogen:The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage:-20°C for 1 year

Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Molecular weight(Da):51590

UniProt accession:Human: P03956

Synonyms:MMP1 ;Cleaved-Ile271; Interstitial collagenase; EC 3.4.24.7; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1; 22 kDa interstitial collagenase; 27 kDa interstitial collagenase;

GeneID:Human: 4312