MASTER MAX™ 1-2-3™ UNIVERSAL PCR BEADS

SKU: PCR-80-08

MASTER MAX™ 1-2-3™ UNIVERSAL PCR BEADS

Product Overview: The MASTER MAX™ 1-2-3™ Universal PCR Beads are meticulously formulated, pre-mixed, and pre-dispensed reagents designed to facilitate efficient and reliable polymerase chain reaction (PCR) amplifications. These beads encompass all essential components, including recombinant HotStart DNA polymerase, deoxynucleotide triphosphates (dNTPs), and a reaction buffer, optimized specifically for endpoint PCR applications. The beads are conveniently available in 0.2 ml 8-strip tubes, making them ideal for a wide array of research applications.

Key Features:

• Enhanced Stability: The beads are stable at room temperature, ensuring a prolonged shelf life and eliminating the need for cold storage.
• User Convenience: Pre-mixed and pre-dispensed format significantly reduces preparation time and minimizes the risk of pipetting errors.
• Versatility: Suitable for a variety of PCR applications, including endpoint PCR and intercalating dye-based assays.
• High Performance: Incorporates high-quality recombinant HotStart DNA polymerase, ensuring robust and reproducible amplification results.

Storage Instructions: Store the beads at ~2-8℃ in an airtight foil pouch containing desiccant. Once the pouch is opened, it should be completely resealed, folded over several times, and secured with a clip. For optimal product longevity, store unopened and resealed pouches in a desiccator.

Components of lyophilized PCR Beads:

• dATP, dCTP, dGTP, dTTP
• ~2.5 units of HotStart DNA polymerase
• Stabilizers
• Reaction buffer

Usage Instructions:

1. Add Primers: Introduce 5'- and 3'-primers (5–25 pmol each) into the reaction tube containing the PCR bead.
2. Add Template: Add the DNA template (50 pg for plasmid DNA or 50 ng for genomic DNA) to the tube.
3. Add Water: Add DNase-free water to achieve a final reaction volume of 25 μl.

See manual for specific details:

Thermal Cycling Conditions:

• Initial Denaturation: 95°C for 6 minutes
• Cycling: 95°C for 20 seconds, 55°C for 20 seconds, 72°C for 30-60 seconds (25-40 cycles)
• Final Extension: 72°C for 10 minutes

Catalog Numbers:

• PCR-80-08: 0.2 ml 8-strip tubes, 48 reactions (6x 8 reactions)
• PCR-80-96: 0.2 ml 8-strip tubes, 96 reactions (12x 8 reactions)

Materials Not Supplied:

• Deionized or Distilled Water: Ensure the water used is nuclease-free and free of contaminating nucleic acids.
• DNA Template: High-quality DNA is recommended for optimal results.
• Template-Specific Primers: Primers should be designed according to the guidelines provided in the product manual.
• Mineral Oil: If required for the thermal cycler being used.

For Research Use Only: These beads are intended solely for research purposes and are not suitable for diagnostic procedures. They should not be used internally or externally in humans or animals.