MAP3K5 Monoclonal Antibody

SKU: BT-MCA3370

Size:100μL

Background:Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

Research_area:Cell Biology

Target_protein:MAP3K5

applications:IHC, ICC, FCM

Reactivity:Human

Clonality:Monoclonal

Clone ID:2E4

Host:Mouse

Isotype:Mouse IgG1

Gene Symbol/ Name:MAP3K5

Immunogen:Purified recombinant fragment of human MAP3K5 expressed in E. Coli.

Storage:4°C|-20°C for long term storage

Purification:

Formulation:Ascitic fluid containing 0.03% sodium azide.

Concentration:

Molecular weight(Da):55kDa

UniProt accession:Q99683

Synonyms:ASK1, MEKK5, MAPKKK5

GeneID:4217