LPS Instant ELISA kit (General species)

SKU: IEB526Ge

Size: 96Tests

# of Times Cited in literature: 7

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: LPS

Target Full Name: Lipopolysaccharide

Alternative Names: LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: IE kit

Featured Series Function: Detects protein (quick procedure in 1 hrs)

Specificity: Reactive with General species LPS / Lipopolysaccharide

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.47-30ng/mL

Sensitivity: 0.21ng/mL

Assay Time: 1h, 10min

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Lipopolysaccharide (LPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Lipopolysaccharide (LPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Instant Lipopolysaccharide (LPS). No significant cross-reactivity or interference between Instant Lipopolysaccharide (LPS) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50μL standard or sample and 50μL Detection Reagent A to each well. Incubate 1 hours at 37°C; 3. Aspirate and wash 3 times; 4. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37°C; 5. Add 50μL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Lipopolysaccharide (LPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Instant Lipopolysaccharide (LPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Instant Lipopolysaccharide (LPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Lipopolysaccharide (LPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: -

References Citing This Product: Relationship between plasma levels of zonulin, bacterial lipopolysaccharides, d‑lactate and markers of inflammation in haemodialysis patients

A combination of Pueraria lobata and Silybum marianum (L.) Gaerth protects against alcoholic liver disease in mice

The Relationship between Low Serum Vitamin D Levels and Altered Intestinal Barrier Function in Patients with IBS Diarrhoea Undergoing a Long-Term Low-FODMAP