GeneBio Systems

Kim1 ELISA kit (Rat)

Name: Kim1 ELISA kit (Rat)
Brand: GeneBio Systems
SKU: SEA785Ra
Price: 157300 JPY
Availability: InStock

SKU:SEA785Ra

¥157,300 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 25

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: Kim1

Target Full Name: Kidney Injury Molecule 1

Alternative Names: HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

Target Species: Rat

Uniprot: O54947

Gene ID: 286934

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat Kim1 / Kidney Injury Molecule 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 62.5-4,000pg/mL

Sensitivity: 24.5pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Urine, Tissue Homogenates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1). No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1 (Kim1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1 (Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney Injury Molecule 1 (Kim1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kidney Injury Molecule 1 (Kim1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Infection immunity;Kidney biomarker;

References Citing This Product: Comprehensive assessment of nephrotoxicity of intravenously administered sodium-oleate-coated ultra-small superparamagnetic iron oxide (USPIO) and titanium dioxide (TiO(2)) nanoparticles in rats.

Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical Model

The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies

Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model

Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats

Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion

Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis

Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis

Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via its antioxidant, anti‑inflammatory, and anti‑apoptotic activities.

Vitamins C and E Alleviate Nephrotoxicity-induced by Potassium Bromate in Rats

Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via itsantioxidant, anti‑inflammatory, and anti‑apoptotic activities.