GeneBio Systems

IL8 ELISA kit (Human)

Name: IL8 ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEA080Hu
Price: 131000 JPY
Availability: InStock

SKU:SEA080Hu

¥131,000 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 50

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: IL8

Target Full Name: Interleukin 8

Alternative Names: CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

Target Species: Human

Uniprot: p10145

Gene ID: 3576

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human IL8 / Interleukin 8

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 15.6-1,000pg/mL

Sensitivity: 6.0pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8). No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 (IL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;Infection immunity;

References Citing This Product: Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa

Experimental peri-implant mucositis at different implant surfaces.

Serum levels of selected chemokines in systemic lupus erythematosus patients

Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination

Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery

The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt

Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period

Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol

Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.

小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响

Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models