IFNa ELISA kit (Human)

SKU: SEA033Hu

Size: 96Tests

# of Times Cited in literature: 22

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: IFNa

Target Full Name: Interferon Alpha

Alternative Names: IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D

Target Species: Human

Uniprot: P01562

Gene ID: 3439

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human IFNa / Interferon Alpha

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 7.8-500pg/mL

Sensitivity: 2.9pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Alpha (IFNa). No significant cross-reactivity or interference between Interferon Alpha (IFNa) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha (IFNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Alpha (IFNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;Infection immunity;

References Citing This Product: The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats

Experimental rat model for alcohol-induced osteonecrosis of the femoral head

Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells

siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro

Sulforaphane Suppresses Hepatitis C Virus Replication by Up-ReCavia (Guinea pig )lating Heme Oxygenase-1 Expressionthrough PI3K/Nrf2 Pathway.

Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells

MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B

Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation.

Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis

Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesis

Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus

Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1

Influenza virus neuraminidase regulates host CD8+ T-cell response in mice

IFN©\¦Á and TNF©\¦Á serum levels and their association with disease severity in Egyptian children and adults with alopecia areata

Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway

Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-¦Ã immunodeficiency: a prospective cohort study

The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides