Human Tumor necrosis factor receptor superfamily member 27 (EDA2R) ELISA Kit

SKU: AE46206HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q9HAV5

Abbreviation: EDA2R

Alternative Names: EDA-A2R; EDAA2R; TNFRSF27; XEDAR; EDA-A2 receptor|X-linked ectodysplasin receptor|X-linked ectodysplasin-A2 receptor|tumor necrosis factor receptor superfamily member XEDAR

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.056 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate EDA2R in samples. An antibody specific for EDA2R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEDA2R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDA2R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDA2R bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. Tumor necrosis factor receptor superfamily member 27 is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Multiple alternatively spliced transcript variants have been found for this gene, but some variants lack sufficient support.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).