Human TRH(Thyrotropin Releasing Hormone) ELISA Kit

SKU: ELK2641

Size:96T

Reactivity:Human

Alternative Names:TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor

Assay Type:Competitive Inhibition

Sensitivity:2.7 pg/mL

Standard:500 pg/mL

Detection range:7.82-500 pg/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Endocrinology;Neuro science;Hepatology;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRH in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P20396