Human Sterol Regulatory Element-binding Protein 1, SREBF1 ELISA Kit

SKU: E6224Hu

Size:96 Tests-1 Kit

Background:[Sterol regulatory element-binding protein 1]: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane (PubMed: 32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis (By similarity). [Processed sterol regulatory element-binding protein 1]: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis (PubMed: 8402897, PubMed: 12177166, PubMed: 32322062). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed: 8402897, PubMed: 12177166). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation (PubMed: 8402897, PubMed: 12177166, PubMed: 32322062). [Isoform SREBP-1A]: Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C (By similarity). Able to stimulate both lipogenic and cholesterogenic gene expression (PubMed: 12177166). Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver (By similarity). Required for innate immune response in macrophages by regulating lipid metabolism (By similarity). [Isoform SREBP-1C]: Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A (By similarity). Primarily controls expression of lipogenic gene (PubMed: 12177166). Strongly activates global lipid synthesis in rapidly growing cells (By similarity). [Isoform SREBP-1aDelta]: The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters. [Isoform SREBP-1cDelta]: The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters.

Research_area:Metabolism

Applications:ELISA

Target_protein:SREBF1

Reactivity:Human

Assay type:Sandwich

Sensitivity:1.75ng/L

Detection range:3-900ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Sterol Regulatory Element-Binding Protein 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P36956

MASS(Da):121,675

GeneID:6720

Synonyms:bHLHd1;Class D basic helix-loop-helix protein 1;Processed sterol regulatory element-binding protein 1;SREBF 1;SREBF1;SREBP-1;Sterol regulatory element-binding protein 1;Sterol regulatory element-binding transcription factor 1;Transcription factor SREBF1;

Gene names:SREBF1

Standard solution:960ng/L