Human Phosphatidylinositol Binding Clathrin Assembly Protein, PICALM ELISA Kit

SKU: E3382Hu

Size:96 Tests-1 Kit

Background:Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly (PubMed: 10436022, PubMed: 16262731, PubMed: 27574975). Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature (PubMed: 25898166). In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8 (PubMed: 22118466, PubMed: 21808019, PubMed: 23741335). In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors (PubMed: 25241929). Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF) (PubMed: 25241929, PubMed: 24067654).

Research_area:Others

Applications:ELISA

Target_protein:PICALM

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.092ng/ml

Detection range:0.2-70ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Phosphatidylinositol Binding Clathrin Assembly Protein

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q13492

MASS(Da):70,755

GeneID:8301

Synonyms:Clathrin assembly lymphoid myeloid leukemia protein;Phosphatidylinositol-binding clathrin assembly protein;PICALM

Gene names:PICALM

Standard solution:80ng/ml