Human NFE2L3(Nuclear factor erythroid 2-related factor 3) ELISA Kit

SKU: ELK9900

Size:96T

Reactivity:Human

Alternative Names:NRF3

Assay Type:Sandwich

Sensitivity:0.06 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Serum, plasma, saliva and other biological fluids.

Assay length:3.5h

Research Area:Epigenetics and Nuclear Signaling; Metabolism

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFE2L3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFE2L3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFE2L3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFE2L3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9Y4A8