Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit

SKU: ELK0857

Size:96T

Reactivity:Human

Alternative Names:RPS12; MRPS12; MRPS34; PSMD7; HTR1B

Assay Type:Competitive Inhibition

Sensitivity:0.28 ng/mL

Standard:50 ng/mL

Detection range:0.79-50 ng/mL

Sample type:cell lysates and other biological fluids

Assay length:2h

Research Area:Developmental science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O15235