Human Mothers Against Decapentaplegic Homolog 4, SMAD4 ELISA Kit

SKU: E0694Hu

Size:96 Tests-1 Kit

Background:In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed: 25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:SMAD4

Reactivity:Human

Assay type:Sandwich

Sensitivity:8.25ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Mothers Against Decapentaplegic Homolog 4

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q13485

MASS(Da):60,439

GeneID:4089

Synonyms:Deletion target in pancreatic carcinoma 4;hSMAD4;MAD homolog 4;Mothers against decapentaplegic homolog 4;Mothers against DPP homolog 4;SMAD 4;SMAD family member 4;Smad4

Gene names:SMAD4

Standard solution:3200ng/L