Human MICB(MHC class I polypeptide-related sequence B) ELISA Kit

SKU: ELK11343

Size:96T

Reactivity:Human

Alternative Names:MHC class I polypeptide-related sequence B;MIC-B;MICB

Assay Type:Sandwich

Sensitivity:11.2 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Immunology

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MICB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MICB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MICB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MICB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q29980