Human Lysophosphatidic acid receptor 1 (LPAR1) ELISA Kit

SKU: AE35306HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q92633

Abbreviation: LPAR1

Alternative Names: EDG2; GPR26; Gpcr26; LPA1; Mrec1.3; VZG1; edg-2; rec.1.3; vzg-1; OTTHUMP00000021904|endothelial differentiation; lysophosphatidic acid G-protein-coupled receptor; 2|ventricular zone gene 1

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.115 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate LPAR1 in samples. An antibody specific for LPAR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPAR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPAR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPAR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Lysophosphatidic acid receptor 1 is a G protein-coupled receptor which binds the lipid signaling molecule lysophosphatidic acid (LPA). Hence this receptor is also known as LPA1.The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Alternative splicing of this gene has been observed and two transcript variants have been described, each encoding identical proteins.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).