Human KLK8(Kallikrein 8) ELISA Kit

SKU: ELK1851

Size:96T

Reactivity:Human

Alternative Names:NP; HNP; NRPN; PRSS19; TADG14; BSP1; Neuropsin; Ovasin; Brain Serine Protease 1; Tumor Associated Differentially Expressed Gene 14; Serine protease 19

Assay Type:Competitive Inhibition

Sensitivity:216.4 pg/mL

Standard:50000 pg/mL

Detection range:781.25-50000 pg/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Enzyme & Kinase;Tumor immunity;Neuro science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK8 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O60259