Human Interferon α (IFN-α) ELISA Kit

SKU: AE38799HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: N/A

Abbreviation: IFN-α

Alternative Names: IFNA

Application: ELISA

Range: 12.5-800 pg/mL

Sensitivity: 6.8 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate IFN-α in samples. An antibody specific for IFN-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIFN-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-α is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-α bound in the initial step. The color development is stopped and the intensity of the color is measured.

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