Human HBg1(Hemoglobin Gamma 1) ELISA Kit

SKU: ELK4070

Size:96T

Reactivity:Human

Alternative Names:HB-G1; HBGA; HBGR; HSGGL1; Gamma-1-globin

Assay Type:Competitive Inhibition

Sensitivity:202.8 ng/mL

Standard:30000 ng/mL

Detection range:468.75-30000 ng/mL

Sample type:Serum, plasma, erythrocyte lysates

Assay length:2h

Research Area:Hematology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBg1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBg1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBg1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P69891