Human GM-CSF ELISA Kit PicoKine®

SKU: EK0364

Size: 96 wells/kit, with removable strips.

Storage: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Form:

Reactivity: Human

Applications: ELISA

Application Details:

Gene Name: CSF2

Specificity: Natural and recombinant human GM-CSF

Background: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

Immunogen: Expression system for standard: E.coli; Immunogen sequence: A18-E144

Clonality:

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Reference:

Uniprot ID: P04141

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Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

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Calculated Molecular Weight:

Gene ID: 1437

Protein Name: Granulocyte-macrophage colony-stimulating factor

Gene Full Name: colony stimulating factor 2

Synonyms: Colony stimulating factor; CSF; CSF2; GM CSF; GMCSF; GM-CSF; Molgramostin; Sargramostim